Abstract
Exploiting heterosis using pollen control systems such as genic male sterility (GMS) can significantly improve the seed yield of sesame. In this study, we developed a novel GMS line (W1098A) by backcrossing and sib-mating the wild accession Yezhi2 (Sesamum mulayanum Nair, 2n = 26) plants with the cultivar Ezhi1 (S. indicum L., 2n = 26). The sterility of this line is mainly controlled by one dominant GMS gene (Ms). Cytological investigation showed that pollen abortion in W1098A plants began in pollen mother cells, continued throughout pollen development, and peaked at the late microspore stage. The abortion process was closely associated with the abnormal behaviour of the tapetum. We employed bulked segregation analysis to identify SSR markers linked to the Ms gene in a segregating population derived from sib-mating between the near-isogenic lines W1098A and W1098B. Among 1,500 SSR primer pairs screened against the sterile and fertile bulks of DNA, 13 markers were found to be linked to the Ms locus. The closest markers on both sides of Ms were SBM298 and GB50, at a respective genetic distance of 0.15 and 0.70 cM. These markers will be valuable for marker-aided breeding of GMS hybrids and map-based cloning of Ms.
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