Abstract
Gel electrophoresis techniques have been used to investigate genetically controlled variation in the major subunits (Mr 50,000) of vicilin, a storage protein of Pisum sativum L. The F1 protein band patterns were shown to be additive with respect to those of the parental lines and to be identical in reciprocal crosses. Genetic analysis of the F2 plants indicated that the 50,000 Mr vicilin subunit band pattern is controlled by a pair of co-dominant genes at single locus. The F2 data were used to locate this major vicilin gene locus (Vc-1) to chromosome 7, closely linked to the r locus (for round and wrinkled seed surface).
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