Abstract
Two Capsicum annuum anthracnose resistant introgression lines PR1 (derived from PBC932C. chinense) and PR2 (derived from PBC80C. baccatum) with resistance conferred by different sources were crossed to a susceptible parent (PS) and F1, F2, BC1PS, BC1PR and F2 of a three-way populations were generated. Fruits of the populations were inoculated with Colletotrichum acutatum-Ca153 using microinjection method at the mature green stage. The SCAR-Indel and SSR-HpmsE032 markers were used for validation in all populations derived from PR1 and PR2, respectively. In both resistance sources (PR1 and PR2), resistance was controlled by a major recessive gene. The resistant banding pattern amplified by SCAR-Indel and SSR-HpmsE032 markers was 100bp and 231bp fragments, respectively. Validation studies of both markers in F2 populations revealed that individually their ability to correctly predict resistant genotypes (selection efficiency) was 65%. However, when both markers were used together, the selection efficiency increased to 77% as well as the efficacy of individual SSR marker used within F2 of the three-way populations.
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