Inhella fonticola sp. nov., isolated from spring water, and emended description of the genus Inhella

Abstract

A yellow-pigmented bacterial strain designated TNR-25(T) was isolated from spring water in Taiwan and was characterized using a polyphasic taxonomic approach. Strain TNR-25(T) was gram-negative, obligately aerobic, rod-shaped, non-motile and non-spore-forming. Growth occurred at 15-40 °C (optimum, 25 °C), at pH 6.0-10.0 (optimum, pH 7.0) and with 0-0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TNR-25(T) belonged to the genus Inhella and its closest neighbour was Inhella inkyongensis IMCC1713(T) with 98.1 % sequence similarity. The major fatty acids (>10 %) of strain TNR-25(T) were summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c) and C(16 : 0). The major cellular hydroxy fatty acids were C(10 : 0) 3-OH and C(12 : 0) 3-OH. The isoprenoid quinone was Q-8 and the DNA G+C content was 69.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and several uncharacterized phospholipids. The DNA-DNA relatedness between strain TNR-25(T) and I. inkyongensis IMCC1713(T) was about 30.6-35.5 %. On the basis of the genotypic and phenotypic data, strain TNR-25(T) represents a novel species in the genus Inhella, for which the name Inhella fonticola sp. nov. is proposed; the type strain is TNR-25(T) ( = BCRC 80211(T) = LMG 25721(T)).