Inhalation Administration of Leytragin to C57BL/6Y Mice in an ARDS Model Increases the Expression Level of SIRT1 Gene

Abstract

This paper describes a technique for inhalation administration of Leutragin into the lungs of C57BL/6Y mice in a model of acute respiratory distress syndrome (ARDS). Inhalations were carried out using an OMRON COMP AIR NE-C24 Kids compression inhaler with a nozzle for simultaneous administration to several mice, developed at the Scientific Center of Biomedical Technologies of FMBA of Russia. Modeling of ARDS was carried out by sequential administration of α-galactosylceramide, inhaled at a dose of 1 μg/mouse, and, following 24 hours, a combination of E. coli lipopolysaccharide (LPS) at a dose of 300 μg/mouse. Thirty minutes after the administration of LPS, inhalation administration of the Leytragin drug was carried out to mice in the experimental group and normal saline in the control group. After inhalation, a biomaterial (lung tissue) was collected to evaluate the expression of the SIRT1 gene by RT- PCR as a marker of successful penetration of the drug into the lung tissue.