Inhalable dust as a marker of exposure to airborne biological agents in composting facilities

Abstract

ObjectivesIndustrial composting is associated with high levels of worker exposure to bioaerosols. Measurement of airborne microorganisms and endotoxin is complex and the related cost is high. The objective was therefore to examine whether dust measurement could be used as a marker of exposure to bioaerosols in composting facilities. MethodsA dataset of 110 measurements carried out in eight sludge composting plants was explored. Mixed-effects models were constructed to explain between-site and within-site variability in concentration of endotoxin and culturable mesophilic bacteria, mesophilic moulds and thermophilic actinomycetes in air. Fixed-effects variables were inhalable dust concentration, the season, the outdoor/indoor location of sampling and the process area. ResultsThe level of dust was a highly significant determinant of concentration for all biological agents. Within-site variability was always larger than between-site variability. The proportion of within-site variability explained by determinants was 68%, 65%, 56% and 60% for endotoxin, bacteria, moulds and actinomycetes, respectively. Inclusion of dust in the final model resulted in an increase of 24, 20, 12 and 17 points of percentage within-site variability, respectively. Inclusion of season resulted in an increase of 9, 12, 12 and 15 points, respectively. Within-site variability was less influenced by outdoor/indoor location and process area, except for moulds. ConclusionDust was the factor that most influenced within-site variability in endotoxin and culturable bacteria concentration. Measurement of dust can efficiently assist decision making for prevention measures against endotoxin and bacteria in sludge composting plants. Our results are not as conclusive for actinomycetes and especially for moulds.