Ingestion of host blood by the monogenean Pseudodiplorchis americanus: a quantitative analysis

Abstract

SUMMARYPseudodiplorchis americanus infects the urinary bladder of a desert toad and feeds exclusively on blood, producing the iron-rich waste product haematin. The host, Scaphiopus couchii, retains dilute urine within the urinary bladder as a water store throughout hibernation and parasite waste will therefore not be eliminated. This study utilized atomic absorption spectrophotometry to quantify the iron within the urinary bladders of infected and uninfected toads after different periods of hibernation. During the first 4 months hibernation, no detectable iron, above that of the controls, was found in the urine and bladder tissue of infected animals, but after 5–6 months hibernation there was a small but not significant increase. The iron contained within most individual parasites was greater than that detected in the urine and bladder tissue of the host, and the total iron in each parasite infrapopulation was 3–83 times greater than the urinary bladder iron. This suggests that the parasites do not regurgitate their gut contents during host hibernation. The amount of iron in individual parasites increased with time after migration to the bladder and this was used to estimate the amount of blood ingested by P. americanus. At a controlled temperature of 25 °C, the estimated rate of ingestion increased from 0·33 μl blood/parasite/week by worms 2 weeks post-migration to a maximum of around 1·6 μl by worms 5 months post-migration. The rate of blood ingestion by older worms was also calculated as a function of the hibernation period (since this did not correspond to worm age) assuming complete gut evacuation at the start of hibernation. This provided maximum estimates of ingestion rate of 1·9–5·3 μl blood/parasite/week.