Ingestion and effects of green synthesized cadmium sulphide nanoparticle on Spodoptera Litura as an insecticidal and their antimicrobial and anticancer activities

Abstract

The current study investigated the multifunctional properties of Cadmium Sulphide Nanoparticles synthesized using a green synthesis method (CdS NPs) using a green feedstock, Nopal Cactus fruit extract. The biological activities of the CdS NPs were thoroughly investigated, including their insecticidal, antibacterial, and anticancer activities. The different concentrations (0.005–0.04%) of CdS NPs were fed to the larvae of Spodoptera litura, and their ingestion effects were observed on the different biological, biochemical, and oxidative stress markers. There are significant dose-dependent changes in the biochemical parameters like superoxide dismutase (SOD), Catalase (CAT), Glutathione-S-transferase (GST), and MDA level as a marker of lipid peroxidation in the treated larvae were studied. In the highest concentration (0.04%), significant larval mortality (46.66%), malformation (pupae and adult) (27.78%), inhibition of adult emergence (43.87%), as well as reduced fecundity (25.28%), and fertility (22.74%) as compared to control was observed. CdS NPs have been investigated for antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus bacterial strains. In vitro anticancer activities were carried out to decrease the viability of the Pancreatic cancer cell line. The cells showed 18% and 12% viability at a 200 μg/ml concentration when incubated with CdS NPs for 24 and 48 h, respectively, confirming its potent anticancer property. The lack of cytotoxicity against the (RBC) endorses the biocompatible nature of synthesized CdS NPs. It was observed that green synthesized CdS NPs could be used as a promising insecticidal, antibacterial, and anticancer agent.