Infusion of Bone Marrow Mesenchymal Stem Cells Attenuates Experimental Severe Acute Pancreatitis in Rats.

Hang Zhao,Jun Gao,Yanfang Gong,Hongyu Wu,Xiangjun Meng,Zhiying He,Dandan Huang,Zhaoshen Li,Aifang Xu

doi:10.1155/2016/7174319

Abstract

Background & Aims. Severe acute pancreatitis (SAP) remains a high-mortality disease. Bone marrow (BM) mesenchymal stem cells (MSCs) have been demonstrated to have plasticity of transdifferentiation and to have immunomodulatory functions. In the present study, we assessed the roles of MSCs in SAP and the therapeutic effects of MSC on SAP after transplantation. Methods. A pancreatitis rat model was induced by the injection of taurocholic acid (TCA) into the pancreatic duct. After isolation and characterization of MSC from BM, MSC transplantation was conducted 24 hrs after SAP induction by tail vein injection. The survival rate was observed and MSCs were traced after transplantation. The expression of TNF-α and IL-1β mRNA in the transplantation group was also analyzed. Results. The survival rate of the transplantation group was significantly higher compared to the control group (p < 0.05). Infused MSCs were detected in the pancreas and BM 3 days after transplantation. The expression of TNF-α and IL-1β mRNA in the transplantation group was significantly lower than in the control group in both the pancreas and the lungs (p < 0.05). Conclusions. MSC transplantation could improve the prognosis of SAP rats. Engrafted MSCs have the capacity of homing, migration, and planting during the treatment of SAP.

Highlights

Acute pancreatitis comprises a mild, self-limiting disease and in about one-third of patients an inflammatory process that causes local and systemic complications, frequently resulting in a systemic organ dysfunction
Serum alanine aminotransferase (AAT) levels were significantly increased at 8 hr after severe acute pancreatitis (SAP), and serum creatinine levels were significantly increased at 24 hrs after SAP (p < 0.05)
The survival rate of the transplantation group was significantly higher compared to the control group (Figure 4(a), p < 0.05)

Summary

Introduction

Acute pancreatitis comprises a mild, self-limiting disease and in about one-third of patients an inflammatory process that causes local and systemic complications, frequently resulting in a systemic organ dysfunction. Despite improvements in diagnostics and therapeutics, the mortality rate of severe acute pancreatitis (SAP) has remained high over the past decade. Systemic inflammatory response syndrome (SIRS) plays an important role in the development of multiple organ dysfunction syndrome (MODS), which is the primary cause of morbidity and mortality. Severe acute pancreatitis (SAP) remains a high-mortality disease. The survival rate was observed and MSCs were traced after transplantation. The survival rate of the transplantation group was significantly higher compared to the control group (p < 0.05). The expression of TNF-α and IL-1β mRNA in the transplantation group was significantly lower than in the control group in both the pancreas and the lungs (p < 0.05). MSC transplantation could improve the prognosis of SAP rats. Engrafted MSCs have the capacity of homing, migration, and planting during the treatment of SAP

Methods

Results

Discussion

Conclusion