Abstract
Infrared spectra of human and animal tissues have been recorded by the methods of attenuated total reflection (both single- and multiple-reflection equipment). Little preparation of the samples is necessary. Single-reflection spectra have shown the application of the method for examining tissues, both normal and diseased. The changes in tissue chemistry produced by the diseased state are evident. Multiple reflection has afforded more intense bands and increased the resolution of the spectra. The tissues examined were human fat, spleen, and aorta, and rat heart, chicken heart, and veal heart (endocardium and atrium). The useful infrared ATR range for tissues has been extended beyond 2000 cm −1 to approximately 3000 cm −1.
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