Abstract
In the present work, the interaction of calcium‐phosphate with DPPC (dipalmitoyl phosphatidylcholine) model membranes has been studied in the presence and absence of vitamin D2 by using Fourier Transform Infrared (FTIR) spectroscopy. Calcium and phosphorus are the most abundant elements in the body. They combine in the form of calcium phosphate salt, called hydroxyapatite. Hydroxyapatite is the major structural component of the bone. Calcium phosphate assists with the digestion and absorption of food and is vitally important for the building of sturdy bone and body structures and a robust constitution. Phosphorus is extracted from foods and its use is controlled by vitamin D and calcium. FTIR spectral analysis results suggested that, calcium–phosphate complex, which is the major component of the bones, decreases the phase transition temperature to lower values, causes a loss in cooperativity of the acyl chains, decreases the order of the membrane in both phases and decreases the dynamics of the membrane in the liquid crystalline phase, increases the flexibility of the chains in the center of the bilayer in both phases, and increases the mobility of the head group of DPPC in the gel phase. The effect of calcium‐phosphate on DPPC liposomes diminishes with the addition of vitamin D2 into the liposomes. Our results suggest how calcium‐phosphate and/or vitamin D2, which have indispensable role for the functioning of the bone tissue, affect the thermal behaviour of DPPC liposomes at molecular level.
Highlights
Phosphatidylcholine (PC) is one of the predominant phospholipid membrane components
Calcium phosphate–vitamin D2–DPPC interactions have been investigated by Fourier Transform Infrared (FTIR) spectroscopy by monitoring different functional groups, such as C–H stretching at 2800–3000 cm−1, C=O stretching at 1735 cm−1 and PO−2 antisymmetric double bond stretching bands at 1220–1240 cm−1
The spectral changes associated with these vibrational modes are of characteristic value, since they can provide valuable structural and conformational information about the thermotropic changes which occur in the acyl chains, interfacial region and the head group region of the lipid molecules, respectively
Summary
Phosphatidylcholine (PC) is one of the predominant phospholipid membrane components. Liposomes of pure dipalmitoylphosphatidylcholine (DPPC) have been widely used as membrane model systems to study the effects of molecules of biological interest (like vitamins and ions) on membrane properties [1].Calcium is the most abundant element in the body [2]. Phosphatidylcholine (PC) is one of the predominant phospholipid membrane components. Liposomes of pure dipalmitoylphosphatidylcholine (DPPC) have been widely used as membrane model systems to study the effects of molecules of biological interest (like vitamins and ions) on membrane properties [1]. Calcium is the most abundant element in the body [2]. Phosphorus, the second most abundant element, combines with calcium in the form of calcium phosphate salt, called hydroxyapatite. Hydroxyapatite is the major structural component of the bone [3]. Calcium phosphate assists with the digestion and absorption of food and is vitally important for the building of sturdy bone and body structures and a robust constitution. Calcium phosphate deficiency is indicated in blood and bone disorders including anaemia [4], osteoporosis [5]
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