Abstract
Abstract Infrared absorptions have been observed in the 1800–700 cm−1 region of four purified amino-acid transfer ribonucleic acids (tRNA’s): alanine tRNA and valine-I tRNA from Torulopsis utilis (torula yeast) and methionine tRNA and tyrosine tRNA from Escherichia coli in their deuterated film. Spectral features in the 1550–1750 cm−1 region of the valine, methionine, and tyrosine tRNA’s indicate that each of these tRNA’s has an amount of base-pairs, which is in accord with the “clover leaf” structure previously proposed. The relative amounts of the paired and unpaired cytosine residues are estimated on the assumption that the intensity ratio of the 1523 and 1500 cm−1bands is 0.18 for the paired cytosine residue and 0.84 for the unpaired cytosine residue. The results are also compatible with the “clover leaf” structure.
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