Abstract
Amplification curves provide information about the kinetics and quality of a quantitative PCR (qPCR) result. An adequate baseline correction is essential for optimal evaluation of the curves. Standard software of the various platforms often under- or overestimate the baseline not only leading to distorted amplification curves but also to wrong values for parameters such as Cq, PCR efficiency and ∆Rn. This chapter describes the fundamentals of the analysis of amplification curves, the corresponding requirements for their quality and some mathematical background. This chapter also gives a short review of various methods to analyse the amplification curves. It is of utmost importance to become aware of the rather large, but artificial, differences in Cq values provided by different methods and platforms.
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