Abstract
OBJETIVO: Verificar a influência do laser de baixa intensidade de AsGaAl na lesão por esmagamento do nervo peroneiro de ratos através da avaliação funcional da marcha nos diferentes locais de irradiação. MÉTODOS: Foram utilizados 53 ratos, divididos em seis grupos: normal, lesado não tratado, lesado e tratado placebo, lesado e tratado na medula, lesado e tratado no nervo, lesado e tratado em ambos (nervo e medula). O esmagamento do nervo peroneiro foi realizado por meio de uma pinça e subsequente tratado com laser por 28 dias consecutivos. Na avaliação funcional foram analisadas as impressões das pegadas, registradas pela câmera de vídeo em uma passarela de acrílico, no pré-operatório e aos 14º, 21º e 28º dias de pós-operatório, e avaliadas na fórmula PFI por meio do software. RESULTADOS: Na avaliação funcional da marcha foram encontradas diferenças significantes somente no 14º dia de pós-operatório. CONCLUSÃO: A irradiação do laser AsGaAl de baixa intensidade foi capaz de acelerar e potencializar o processo de regeneração nervosa periférica de ratos no 14º dia de pós-operatório, de acordo com a avaliação funcional da marcha, tanto para o grupo tratado na medula quanto para o grupo tratado no nervo.
Highlights
Several authors investigate the influence of the various therapeutic treatments, in the regeneration of rat sciatic nerve, such as electrical stimulation,[1] therapeutic ultrasound[2] and low-intensity laser that has received more attention in the last decade.[3]
Rochkind et al.[4] used laser irradiation at the root of the spinal cord, in the branch that corresponds to the sciatic nerve of rats (L2), and were able to observe an increase in neuron metabolism and an improvement in myelin production that serves to accelerate injured nerve regeneration
The animals were divided into six experimental groups, depending on the procedure performed: GROUP 1: normal, uninjured and untreated (n=5); GROUP 2: injured and untreated (n=10); GROUP 3: injured and treated using placebo (n=8); GROUP 4: injured and treated in the spinal cord, in the region that corresponds to the root of the sciatic nerve, by 4 points between vertebrae T12-T13-L1 along the segments of the spinal cord from L3-L6 (n=10); GROUP 5: injured and treated on the right lower limb surrounding the scar, by 4 points (n=10); GROUP 6: injured and treated on both; two points were divided for each region, whereas the points corresponded to L2 in the spinal cord and on the lower limb at the scar extremities (n=10)
Summary
Several authors investigate the influence of the various therapeutic treatments, in the regeneration of rat sciatic nerve, such as electrical stimulation,[1] therapeutic ultrasound[2] and low-intensity laser that has received more attention in the last decade.[3]. Rochkind et al.[4] used laser irradiation at the root of the spinal cord, in the branch that corresponds to the sciatic nerve of rats (L2), and were able to observe an increase in neuron metabolism and an improvement in myelin production that serves to accelerate injured nerve regeneration. Anders et al.[3] explained that the effectiveness of low-intensity laser irradiation in peripheral nerve recovery can be increased if the corresponding segment of the spinal cord is irradiated. The aim of this study is to verify the influence of the low-intensity GaAsAl (830nm) laser beam on a crush injury of the common peroneal (common fibular) nerve of rats, through functional gait assessment at the different irradiation sites
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
