Abstract
Cytoplasmic extracts of influenza virus-infected BHK21-F and MDBK cells were separated by equilibrium sedimentation into fractions containing smooth membranes, rough membranes, and free ribosomes and polysomes. Analysis by polyacrylamide gel electrophoresis revealed that viral polypeptides were associated with all cytoplasmic fractions. Smooth membranes contained large amounts of viral glycoproteins, as well as the nonglycosylated polypeptide (M) thought to be associated with the viral membrane. Rough membranes also contained the uncleaved hemagglutinin glycoprotein (HA), and results of pulse-chase experiments suggest that this polypeptide is synthesized in association with the rough membranes and accumulates in smooth membranes. The nucleoprotein subunit NP was located mainly in the soluble fraction as well as a cell fraction of intermediate density. The nonstructural viral polypeptide NS (∼25,000 MW) appeared to be associated specifically with ribo-some-containing fractions.
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