Abstract
To determine whether nonhuman primates are infected with influenza viruses in nature, we conducted serologic and swab studies among macaques from several parts of the world. Our detection of influenza virus and antibodies to influenza virus raises questions about the role of nonhuman primates in the ecology of influenza.
Highlights
The WHO Collaborating Centre for influenza at CDC Atlanta, United States of America, has made available the protocol, attached, of realtime RTPCR for swine influenza A(H1N1)
Assay principle: The CDC Realtime RTPCR Protocol for Detection and Characterization of Swine Influenza includes a panel of oligonucleotide primers and dual®
Labeled hydrolysis (Taqman ) probes to be used in real-time RT-PCR assays for the in vitro qualitative detection and characterization of swine influenza viruses in respiratory specimens and viral cultures
Summary
Because of the sensitivity of fluorogenic 5’ nuclease assays, special precautions must be taken to avoid false positive amplifications. (a) Maintain separate areas for assay setup and handling of nucleic acids. (b) Maintain separate, dedicated equipment (e.g., pipettes, microcentrifuges) and supplies (e.g., microcentrifuge tubes, pipette tips) for assay setup and handling of extracted nucleic acids. (c) Wear a clean lab coat and powder-free disposable gloves (not previously worn) when setting up assays. (d) Change gloves between samples and whenever you suspect they may be contaminated. (e) Keep reagent and reaction tubes capped or covered as much as possible
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