Abstract
The protein fraction induced by the WSN strain of influenza virus, which has a molecular weight of about 25,000, was isolated from infected chick embryo fibroblasts by extraction into acidic chloroform-methanol or by column chromatography with 6 M guanidine hydrochloride. The isolated material was analyzed in two dimensions by peptide mapping and the results showed the presence of membrane (M) and the nonstructural (NS) proteins. Both proteins are associated with isolated nuclei, cytoplasm, and polysomes and are distinct proteins. Subfractionation of nuclei indicated that NS was associated with nucleoli or components cosedimenting with nucleoli whereas M remained in the nucleoplasm.
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