Influencing of venom nerve growth factor on expressing of extracellular signal-regulated kinase and nuclear factor-κB in rat brain tissue after cerebral ischemia reperfusion injury

Abstract

Objective To investigate the significance and mechanism of Intracerebroventricular injection viper venom nerve growth factor (vNGF) in rat neural plasticity after cerebral ischemia reperfusion injury. Methods Ninety wistar male rat were randomly assigned into vNGF-25u group ( n = 18), vNGF-50u group(n = 18), vNGF-100u group(n = 18), ischemia reperfusion (I/R) group(n = 18) and sham operated(s) group. The expression of extracellular signal-regulated kinase (ERK) mRNA and nuclear factor-κB (NF-κB) mRNA in rat brain tissues which were collection at 2, 7, 14 days after surgery were evaluated by the Real-time polymerase chain reaction (PCR). Results ( 1 ) The expression NF-κB mRNA began to increase after surgery, while ERK mRNA decreased. At the same time, expression of NF-κB mRNA in the vNGF groups more than in I/R group. The more vNGF were injected, the more NF-κB mRNA were expressed. (2) The expression of ERK mRNA in I/R control group more than other groups.Conclusion The vNGF could accelerate neural plasticity and restore neurofunctional defect through up-regulated the expression of NF-κB and down-regulated the expression of ERK. Key words: Brain ischemia reperfusion; Real-time PCR; vNGF; ERK; NF-κB