Influences of wheat bran fiber on growth performance, nutrient digestibility, and intestinal epithelium functions in Xiangcun pigs

Abstract

Dietary fiber (DF) has long been looked as an essential “nutrients” both for animals and humans as it can promote the intestinal tract development and modulate the intestinal epithelium functions and the gut microbiota. This study was conducted to investigate the influences of wheat bran fiber (WBF) on growth performance and intestinal epithelium functions in Xiangcun pigs. Twenty Xiangcun pigs with 60 days of age were divided to two groups and exposed to a basal diet (BD) or BD containing 4.3% wheat bran fiber (WFD). WFD improved the average daily gain (ADG) and feed-to-gain ratio (F:G) (p < 0.01). Moreover, WFD lowered the serum triglyceride (TC), d-lactate, and malonicdialdehyde (MDA) concentrations, but significantly improved the glutathione (GSH) activity and total antioxidant capacity (T-AOC) (p < 0.05). Interestingly, WFD observably improved the villus height (VH) and the villus height to crypt depth ratio (V/C) in the small intestine (p < 0.05). The jejunal sucrase and ileal maltase activities were higher in the WFD group (p < 0.05). WFD markedly elevated the tight junction protein ZO-1 and claudin-1 expression levels in the jejunum and ileum (p < 0.05). The sodium/glucose co-transporter 1 (SGLT1), glucose transporter 2 (GLUT2), and fatty acid transport proteins 4 (FATP-4) expression levels in jejunum and ileum were also elevated under WFD (p < 0.05). WFD decreased the IL-6 impression level in the duodenum and ileum, but significantly increased the IL-10 expression levels in jejunum and ileum (p < 0.05). Moreover, WFD reduced the abundance of E. coli, but elevated the abundances of beneficial microorganisms (e.g. Lactobacillus and Bacillus) and the production microbial metabolites (e.g. propionic acid and butyrate acid) in the cecum (p < 0.05).