Influences Of Swimming On Learning And Memory And Expression Of Orexin And Ncam In Rat Hippocampus

Abstract

PURPOSE: Through the different load intensity swimming exercise intervention in rats, we observed that the movement influenced on rats spatial learning and memory ability and detected the proteins expression of rat hippocampal. METHODS: 30 male SD rats were selected. After acclimatization to the laboratory for 1 week, the rats were randomly divided into 3 groups: Control Group(C), Medium load Group (M), over load Group (O).Group C were feed naturally; group M did swimming exercise intervention; group O did over load swimming exercise. The Morris Water Maze test (MWM) was performed to evaluate spatial learning and memory ability. Tissue samples were collected after the MWM test. 6 whole brain samples for immunofluorescence assay, while the bilateral hippocampus of the other 6 brains for western blot and real-time PCR analysis. RESULTS: (1) Blood testosterone detections: there was no significantly difference between group M and C (P>0.05), the blood testosterone of group O was significantly lower than group M and C (P<0.01); (2) MWM detection: during the navigation experiment, group M was easier to find the platform than group C (P<0.01). During the space exploration, rats in group M crossed where the platform was fixed more often than group C and O(p < 0.05),rats in group C crossed where the platform was fixed more often than group O(p < 0.05); (3) mRNA detection: the expression of NCAM mRNA and OXA mRNA of group M were significantly higher than group C (P<0.05), OX1R mRNA of group M was significantly lower than group C(P<0.05); (4) Protein detection: the expression of NCAM of group M was significantly higher than group C(P<0.05),the group O was significantly lower than group C(P<0.05). CONCLUSION:Medium load swimming exercise could improve the rats spatial learning and memory ability and significantly improve the expression of OXA, OX1R mRNA and OX1R, and significantly reduce the expression of OXA, OX1R mRNA, OX1R; Over load swimming exercise could damage the spatial learning and memory ability of rats, after over load swimming exercise, the expression of NCAM, OXA and OX1R was normal. Acknowledgments: Supported by the National Natural Science Foundation of China (No.31371202). Supported by the Sports Medicine key laboratory of State Administration of Sports/ Sichuan province Foundation.