Abstract
The surfactant Tween 80 was evaluated for its ability to influence cumulative gas production, cellulose digestion, and enzyme activities by mixed ruminal microorganisms grown on barley grain or Orchardgrass hay. The addition of Tween 80 at a level of 0.10% significantly (p<0.05) decreased the cumulative gas production rate from both barley grain or Orchardgrass hay substrates. However, 0.05% Tween 80 did not affect gas production rates compared to the control treatment. The addition of 0.05% Tween 80 to cultures growing on barley grain resulted in a significant increase in cellulase (90.01%), xylanase (90.73%) and amylase (487.25%) activities after 30 h incubation. Cultures utilizing Orchardgrass hay had a significant increase in cellulase (124.43%), xylanase (108.86%) and amylase (271.22%) activities after 72 h incubation. These increases in activities were also observed with cultures supplemented with 0.10% Tween 80 throughout all the incubation times tested. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some of key enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our data indicates potential uses of the surfactant Tween 80 as a feed additive for ruminant animals. (Asian- Aust. J. Anim. Sci. 2003. Vol 16, No. 8 : 1151-1157)
Highlights
0.05% Tween 80 did not affect gas production rates compared to the control treatment
(108.86%) and amylase (271.22%) activities after 72 h incubation. These increases in activities were observed with cultures supplemented with 0.10% Tween 80 throughout all the incubation times tested. These results indicated that the addition of 0.05% Tween could greatly stimulate the release of some of key enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism
The cumulative gas addition of 0.1% Tween 80 significantly (p
Summary
Was included at a concentration of 0.05% (v/v) in the growth medium, this material increased the growth rate of rumen bacteria and fungi, and the rate of cereal grain digestion, succinate and lactate dehydrogenase activities, and polysaccharide-degrading enzymes activities (Lee et al., 2003). The effects of Tween 80 on the cumulative gas production and enzyme activities by mixed rumen microorganisms are reported here. Considerable efforts have been devoted to manipulating the rumen environment with the aim of enhancing feedstuff utilization and improving the efficiency of ruminant production. The results of these efforts are a wide range of feed additives that are capable of influencing some component of rumen metabolism. We found that this material might be of use as an alternative feed additive that stimulates succinate and lactate utilization by rumen microorganisms in cattle fed high-grain diets, and as an inducer of polysaccharide-degrading enzymes activities.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
