Influences of intrathecal administration of roscovitine on bone cancer pain in mouse models

Abstract

Objective To investigate the influences of intrathecal administration of roscovitine on bone cancer pain in mouse models. Methods Twenty four C3H/Hej mice were divided randomly into group S(sham + vehicle ), group C(tumor + vehicle) or group R (tumor+ roscovitine) (n = 8 ). Osteosarcoma NCTC 2472 cells were implanted into the intramedullary space of the right femurs of mice to make bone cancer pain models. α-MEM (group Sand group C) or roscovitine (group R) was injected in 14 post-implanted day. Pain behaviors such as paw withdrawal mechanical threshold(PMWT) and paw withdrawal thermal latency (PWTL) were observed at 1,6, 24,48 and 72 post-injective hour, respectively. Results The level of PMWT and PTWL among every group had no difference before operation. At day 7 after inoculation, PMWT of group tumor decreased to (1.08±0.24) g, and at day 10 PTWL shortened to (12.7±1.4) s, and then the pain behaviors of group tumor showed aggravation along with the development of cancer pain. There was significant difference in the pain behavior thresholds between group sham and group tumor (P＜0.05). The level of PM WT [ (0.70±0.19 ) g ] and PTWL [ ( 14.16±1.07 ) s] increased compared with group C and base level at 6 h after intrathecal administration of 20 μg Roscovitine, and kept increasing until reaching maximum level after 12 h, then the value decreased gradually, lowering to the level of group C after 72 h. Conclusion Intrathecal administration of roscovitine can relief bone cancer pain in mouse models. Key words: Cdk5; Cancer pain; Roscovitine