Influences of flurbiprofen axetil on the kidney, platelet aggregation and coagulation function in rats with bone cancer

Abstract

Objective To investigate influences of flurbiprofen axetil on kidney,platelet aggregation and coagulation function.Methods Thirty-six healthy female Sprague Drawley (SD)rats were randomly divided into six groups (6 rats in each group):cancer+normal saline group(CN),cancer+flurbiprofen axetil 10 mg/kg group(CK10),cancer+flurbiprofen axetil 25 mg/kg group (CK25),cancer+ flurbiprofen axetil 50 mg/kg group (CK50),flurbiprofen axetil 50 mg/kg group (K50),and sham+normal saline(sham).Flurbiprofen axetil or normal saline was administered intraperitoneally twice a day from day 14 to day 21 after injection of Walker256 tumour cells into one tibia.Rats were sacrificed to obtain blood from abdominal aorta on the 21th day.Then the levels of blood urea nitrogen (BUN),creatinine (Cr),sodion,Potassium ion,protrombin time(PT),activated partial thromboplastin time (APTF),fibrinogen (Fib),platelet aggregation (PA) were detected and pathological changes of kidney were observed.Results Respectively compared with the sham and C groups,the levels of BUN in the CK50 (9.9 ± 1.5) mmol/L and K50 (9.7±1.4) mmol/Lgroups increased obviously 7 days after intraperitoneal injection of flurbiprofen axetil (P＜0.05),and the levels of sodion in the CK50(137±8) mmol/L and K50 (138±8) mmol/L groups and potassium ion in the CK50 (3.9±0.3) mmol/Land K50 (3.9±0.4) mmol/Lgroups reduced significantly (P＜0.05),and the levels of Cr,PT,APTT,Fib and PA were no statistical difference (P＞0.05).Respectively compared with the sham and C groups,the levels of Cr,BUN,sodion,Potassium ion,Cr,PT,A PTT,Fib and PA were no statistical difference obviously in the CK10 and CK25 groups(P＞0.05).The main pathological changes in the CK50 and K50groups were glomerular narrow and poor supplement with blood in capillaries,while in the sham,C,CK10 and CK25 groups,there were no pathological changes observed.Conclusions Intraperitoneal injection of flurbiprofen axetil in a certain range of dose was not found to impair platelet aggregation and coagulation function in a model of bone cancer pain in the rat.However,in large dose,there were obviously influences on the metabolism of BUN,sodion and potassium ion as well asinfusion of capillaries of renal glomeruli in rats. Key words: Flurbiprofen axetil; Kidney; Platelet aggregation; Coagulation function