Influences of Ethyl Acetate and Water Bulb Extracts of Crinum zeylanicum (Linn.) on Haematological Constituents of Rabbit Mediated Through Osteotomy

Abstract

Crinum zeylanicum bulb is a traditional folk’s herb that has been widely accepted by most rural communities for the treatment of bone fracture and other illnesses. This study assessed the influences of C. zeylanicum bulb extracts on blood pictures of rabbits through close tibia osteotomy over the period of three months. Assay of the whole blood collected through cardiac puncture was done by determining the Packed Cell Volume (PCV%) (Micro-hematocrit method), White Blood Cell (WBC%), Red Blood Cell (RBC%) (Hemo- cytometer method), Haemoglobin (HB g/dl) (Cyanomoglobin method), Neutrophil (N%), Lymphocyte (L%), Monocyte (M %), Eosinophil (E%), and Basophil (B %) (Thin smear and Leishman’s Methods). The Mean Cell Volume (MCV%), Mean Corpuscular Hemoglobin (MCH %), and MCH-Concentration (MCHC %) were calculated according to standard methods. There was significant difference (p<0.05) recorded for MCV, MCH, N and L for treated-groups as compared with WBC, MCV and N for control group while the resultant increase of WBC by ethyl acetate extract (EAE) treated group than water extract (WE) and control groups was an indication that the plant has influence on the blood constituents. M, B and E were not dictated during the pre- and post-examinations. Hence, the use of C. zeylanicum can be monitored, modified, and/or regulated for medicinal uses as the characterization of the chemical components of the plant could be a better focus for further investigation.