Abstract
Nanozyme-based colorimetric sensors have received considerable attention due to their unique properties. The size, shape, and surface chemistry of these nanozymes could dramatically influence their sensing behaviors. Herein, a comparative study of VO2 nanoparticles with different morphologies (nanofibers, nanosheets, and nanorods) was conducted and applied to the sensitive colorimetric detection of H2O2 and glucose. The peroxidase-like activities and mechanisms of VO2 nanoparticles were analyzed. Among the VO2 nanoparticles, VO2 nanofibers exhibited the best peroxidase-like activity. Finally, a comparative quantitative detections of H2O2 and glucose were done on fiber, sheet, and rod nanoparticles. Under the optimal reaction conditions, the lower limit of detection (LOD) of the VO2 nanofibers, nanosheets, and nanorods for H2O2 are found to be 0.018, 0.266, and 0.41 mM, respectively. The VO2 nanofibers, nanosheets, and nanorods show the linear response for H2O2 from 0.025–10, 0.488–62.5, and 0.488–15.625 mM, respectively. The lower limit of detection (LOD) of the VO2 nanofibers, nanosheets, and nanorods for glucose are found to be 0.009, 0.348, and 0.437 mM, respectively. The VO2 nanofibers, nanosheets, and nanorods show the linear response for glucose from 0.01–10, 0.625–15, and 0.625–10 mM, respectively. The proposed work will contribute to the nanozyme-based colorimetric assay.
Highlights
Natural enzymes with great catalytic capacity and high substrate specificity have attracted much research interest in the fields of medicine, biology, and food industry
Artificial mimic enzymes have the characteristics of high catalytic efficiency, stability, economy, and large-scale preparation which has been rapidly developed in the fields of medicine, chemical industry, food, agriculture, environmental science, and analytical chemistry [2]
Many nanoparticles have been studied as enzyme mimetics, including ferromagnetic NPs [3,4,5,6,7,8,9,10,11], cerium oxide NP [12,13,14], metal NPs [15,16,17,18,19,20,21,22,23], carbon-based nanomaterials [24,25,26,27,28], V2O5 nanowires [29,30], and perovskite oxide [31,32]
Summary
Natural enzymes with great catalytic capacity and high substrate specificity have attracted much research interest in the fields of medicine, biology, and food industry Despite these broad developments, natural enzymes often have inherent drawbacks, such as high preparation and purification costs, low operational stability, sensitivity of catalytic activity to environmental conditions, and difficulty of recovery. TMNBanoamsattehriealss2u01b7s, t7r, a34t7e was apparently lower than VO2 nanofibers, VO2 nanorods, V2O5 nanoz5yomf 10es, Fe3O4 MNPS, and HRP. It shows that the VO2 nanosheets have a higher affinity to TMB compared with VOV2On2annaonfiobroedrss,, VVO2O25 nnaannoorzoydmse,sF, eF3eO3O44MMNNPPSS,, aannddHHRRPP..IWt shhiocwhsmtheaatntshtehVatOa2 lnoawnoesrhTeeMtsBhcaovnecaenhtirgahteiron wnaanavlsofaofwlrifiuneebeqirtseyuTroiMtsrofewBTdVMciOtothoB2ncHncreoean2mantOcropahf2aitbiratoeehsnrdestwwhmwaeitsiahtsrhxueViqbmHOus22atiOrrnlea2adatneactostowifvirtbheaietaesyrcsahs,fupVotbhprOseatV2rrmnaeOtaanenx2tilowmnyraoaalsdnol saowa,cspFtehiperve3aiOterthyet4snaMf.ntolrNyTVVhPlOOoSe,w22aanennpradapntnHaohorsaRehrnPone.edtVWtssKO,.hM2TVichnOhveaa2nmalopnuerpaaoeanndsrsoseo,tnshfhtVaVKeOteMOa2ts, Fe3nOan4oMshNeePtsS,, Faen3Od4HMRNP.PISt, sahnodwHsRtPh.atItthsheoVwOs 2thnaatntohfie bVeOrs hnaavneofaibherisghhearveafafinhiitgyhefor raHffi2nOity cfoormHp2aOre d witchomVpOa2rendanwoisthheVetOs,2VnOan2onsahneeotrso, dVs,OF2en3Oan4oMroNdsP, SF,ea3nOd4 HMRNPP.ST, haantdmHeaRnPs. aThloawt emreHan2sO2a cloonwceerntHra2Otio n wacsornecqenutirraetdiotnowreaascrheqtuhieremd atoxirmeaachl athcteivmitayxifmoralVaOcti2vnitaynfoorfiVbOer2sn.
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