Influence of varying intensities of water-jet force on autologous fat draft viability: an in-vitro study

Abstract

Objective To observe the effect of varying intensities of water-jet force on autologous fat graft viability. Methods Lipoaspirate was taken from 12 female patients undergoing water-jet assisted abdominal liposuction at our department. According to the intensity of water-jet force, the experimental group was divided into four subgroups: R1 (pressure, 30 bar), R2 (pressure, 50 bar), R3 (pressure, 70 bar) and R4 (pressure, 90 bar). Hand-held suction was taken as the control group C. Adipose tissue was filtered with cotton cushion and centrifuged at low speed, and the composition ratio of water and fat tissue from each group was observed. Calcein-AM/Hoechst 33342 staining was used to detect the viability of adipocytes. Results Fat aspirates was divided into four layers: oil layer, pure fat tissue, liquid and bottom sediment. Oil ratios of R1, R2, R3, R4 and C were (8.9±2.3)%, (9.6±2.1)%, (10.3±1.3)%, (14.2±1.6)% and (9.5±1.8)%, respectively. There was no statistically significant difference between R1, R2, R3 and C (P>0. 05). Statistically significant difference was found between R4 and other groups (P 0.05). Statistically significant difference was found between R3, R4 and C (P<0.05). Conclusions Viability of fat graft harvested under lower intensity of water-jet force (R1, R2) is higher than that harvested under higher intensity of water-jet force (R3, R4). Key words: Breast Beauty; Transplantation; Abdominal fat; Water-jet system; Calcein-AM/Hoechst 33342 staining; Intensity of water-jet force