Influence of UV-B radiation and Cd2+ on chlorophyll fluorescence, growth and nutrient content in Brassica napus

Abstract

The possible interaction of two stresses, UV-B radiation and cadmium, applied simultaneously, was investigated in Brassica napus L. cv. Paroll with respect to chlorophyll fluorescence, growth and uptake of selected elements. Plants were grown in nutrient solution containing CdCl 2 , (0, 0.5, 2 or 5 μM) and irradiated with photosynthetically active radiation (PAR, 400-700 nm, 800 μmol m -2 s -1 ) with or without supplemental ultraviolet-B radiation (UV-B, 280-320 nm, 15 kJ m -2 d -1 , weighted irradiance). After 14 d of treatment, the most pronounced effects were found at 2 and 5 μM CdCl 2 with and without supplemental UV-B radiation. Exposure to cadmium significantly increased the amount of Cd in both roots and shoots. In addition, increases occurred in the concentration of Fe, Zn, Cu, and P in roots, while K was reduced. In shoots the S content rose significantly both in the presence and absence of UV-B radiation, while significant increases in Mg, Ca, P, Cu, and K occurred only in plants exposed to Cd and UV-B radiation. Manganese decreased significantly under the combined exposure treatment. The rise in S content may have been due to stimulated glutathione and phytochelatin synthesis. Cadmium exposure significantly decreased root dry weight, leaf area, total chlorophyll content, carotenoid content, and the photochemical quantum yield of photosynthesis. As an estimation of energy dissipation processes in photosynthesis, non-photochemical quenching (q NPQ ) was measured using a pulse amplitude modulated fluorometer. The q NPQ increased with increasing Cd, while the combination of cadmium and UV-B reduced the q NPQ compared to that in plants exposed only to cadmium or UV-B radiation. The chlorophyll a:b ratio showed a reduction with UV-B at no or low Cd concentrations (0 μM, 0.5 μM CdCl 2 ), but not at the higher Cd concentrations used (2 μM, 5 μM CdCl 2 ). Thus in some instances there appeared to be a UV-B and Cd interaction, while in others plant response could be attributed to either treatment alone.