Abstract
In this study, 20 Listeria monocytogenes isolates detected in a food processing environment and food products were tested for biofilm-forming ability in two different culture media: Tryptone Soya Broth and Luria Bertani Broth. Statistical analysis of the data obtained was performed with the MINITAB software package, version 16.0. The two-sample t-test and confidence interval were used for data analysis. Significant differences between the isolates were observed in the ability to form biofilms.
Highlights
A foodborne pathogen Listeria monocytogenes is the causative agent of listeriosis, a severe disease with high hospitalisation and case fatality rates
Our results demonstrated a strong influence of nutrient availability on biofilm production by L. monocytogenes
Box plots were used to illustrate both the measures of central tendency and the variability of the data on the distribution of biofilm formation by L. monocytogenes isolates grown in Tryptone soya broth (TSB) and Luria Bertani (LB) medium (Figure 1)
Summary
A foodborne pathogen Listeria monocytogenes is the causative agent of listeriosis, a severe disease with high hospitalisation and case fatality rates. It can survive and grow in a wide range of adverse environmental conditions typical of food processing and preservation [1]. Having colonised the food processing environment, L. monocytogenes can spread throughout the facility via aerosols, personnel, food workflows and contaminated contact materials leading to its persistent presence if sanitation procedures are insufficient [3]. The aim of this study was to investigate the influence of two different culture media on biofilm formation by L monocytogenes isolates from a small-scale meat processing facility. The susceptibility of the isolates to several antibiotics was assessed
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