Abstract
Study objective:This study reports on marker profiling changes of normal keratinocytes under influence of mesenchymal cells derived from tumor stroma in vitro, and especially on induction of stem‐cell‐like features in affected epithelia.Methods:We compared phenotype of human tumors with in vitro model where growth and phenotypic pattern of normal human keratinocytes were monitored under influence of various types of fibroblasts including those prepared from stroma of malignant tumors. We visualized panel of keratins, endogenous lectins and binding sites for endogenous lectins, proliferation marker Ki67, membrane protein Ber‐EP4, nucleolar protein nucleostemin and markers of poorly differentiated embryonic stem cells Oct‐4 and Nanog.Results:The results demonstrated that normal human keratinocytes were strongly influenced by tumor stromal cells. The observed epithelial cells subsequently expressed tumor markers as keratin 8 and epithelial antigen Ber‐EP4 as well as some putative stem cell markers (e.g. keratin 19, nucleostemin etc.)In conclusion, the cancer stromal cells significantly influenced the phenotype of normal keratinocytes, suggesting so the role of epithelial – mesenchymal interaction in cancer cells survival and spreading.
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