Abstract
Influence of tryptophan on auxin-synthesizing ability of surfactant producer Acinetobactercalcoaceticus IMV B-7241
Highlights
The aim of this work is to determine the optimal tryptophan concentration and the time of its addition into the culture medium of the surfactant producer Acinetobacter calcoaceticus IMV B-7241 to achieve maximum auxin synthesis
The results show that regardless of the time of addition of tryptophan in the culture medium of the strain IMV B-7241 with crude glycerol a significant increase in the synthesis of auxins compared with those on the medium without this precursor was observed
Increased synthesis of auxins by strain IMV B-7241 correlated with the activity of tryptophan transaminase: under cultivation on crude glycerol without precursor it was 163 nmol·min-1·mg-1 of protein, while in the presence of 300 mg/L of tryptophan added at the end of the exponential growth phase the activity was in 3.2 times higher – 526 nmol·min-1·mg-1 of protein
Summary
The aim of this work is to determine the optimal tryptophan concentration and the time of its addition into the culture medium of the surfactant producer Acinetobacter calcoaceticus IMV B-7241 to achieve maximum auxin synthesis. Previous studies have established the ability of the producer of surfactants Acinetobacter calcoaceticus IMV B-7241 to synthesize stimulatory phytohormones – auxins, cytokinins and gibberellins [1]. Such results are the basis for the development of complex preparations with growth-stimulating and antimicrobial activities against phytopathogenic bacteria properties for use in plant production. The following studies [2, 3] conducted at the greenhouses of the D.K. Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine using as test cultures of tomatoes and barley, confirmed that the supernatant of the culture broth A. calcoaceticus IMV B-7241 has a positive effect on plant growth and development. In the case of treatment of the root system of tomatoes with a solution of phytohormones A. calcoaceticus IMV B-7241, the number of fruits exceeded the control by 2.4 times and the total weight by 145.0%
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