Abstract
1. 1. Tricaine methane sulfonate (Sandoz, MS 222) is an immersion anesthetic commonly employed in work of amphibians. 2. 2. Twenty-four hours after anesthetization with this drug Bufo marinus toads have lost an average of 8·2 per cent of their body weight with a concomitant increase of the plasma sodium concentration by about 8 per cent. 3. 3. Plasma sodium concentration begins to return to normal after 2–3 days after 7 days no consistent change is found. 4. 4. These changes seem to involve water balance primarily and it is unnecessary to hypothesize alterations in sodium balance caused by this drugs.
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