Influence of trees on the spatial structure of arbuscular mycorrhizal communities in a temperate tree-based intercropping system

Abstract

Tree-based intercropping (TBI) is an ecologically sustainable agricultural practice that may promote a more diverse arbuscular mycorrhizal (AM) fungal community compared to conventional systems, but the influence of the dynamics of these systems on AM fungi has not been established. Soil and root samples were collected in the intercropping alleys along transects perpendicular to tree rows occupied by white ash ( Fraxinus americana), poplar ( Populus deltoids × nigra), Norway spruce ( Picea abies), and rows without trees (control). Molecular analysis of the AM fungal community at the TBI site revealed 17 phylotypes belonging to the Glomeraceae. Overall, the AM fungal community in the TBI site was comparable to other conventional agricultural systems; with the majority of phylotypes belonging to Glomus group A. AM fungal phylotype richness and community composition significantly differed among the treatments in the TBI site. AM fungal communities were more diverse in cropping alleys adjacent to trees that associate with AM fungi than trees that do not associate with AM fungi. Norway spruce had a negative influence on the AM fungal community as tree rows and bordering intercropping alleys had a significantly lower phylotype richness and different community composition. These results suggest that to maintain a diverse AM fungal community throughout TBI systems, it may be best to incorporate tree species that associate with AM fungi.