Abstract
The effect of the addition of titanium ion (Ti) on osteoblast function and the mineralization of osteoid nodules in rat calvaria cultures was characterized. Concentrations of 10 ppm of Ti or more inhibited cell proliferation; 5 ppm or less either had no effect or stimulated proliferation. The number of nodules formed was not influenced by 5 ppm of Ti, but mineral deposition in nodules was suppressed, as revealed by von Kossa staining. Likewise, 5 ppm of Ti inhibited the incorporation of [45Ca] in cultures during nodule formation even if the Ti was withdrawn from the medium when mineralization was initiated. In order to test whether the synthesis of osteoid components was affected, the expression of osteonectin (OSN), osteopontin (OPN), osteocalcin (OSC), and alkaline phosphatase (ALP) mRNAs as well as ALP enzyme activity was analyzed. The expression of OSN and OPN mRNAs was reduced dramatically, but OSC mRNA was little affected by 5 ppm of Ti. Ti delayed the development of ALP mRNA expression and enzyme activity relative to the controls. Thus Ti treatment changed the proportional composition of cellular mRNA contributing the osteoblast phenotype. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 47, 220–227, 1999.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
