Influence of Thrombophilic Genes; MTHFR (C677T), FVL (G1691A) and ACE (I28005D) In Pregnant Women with Pre-Eclampsia

Abstract

Objective: Pre-eclampsia (PE) is a pregnancy complication and vascular disorder recognized by new onset gestational hypertension and proteinuria. It may lack of immunological mother and fetus signalization. The exact pathogenesis of PE remains uncertain. The aim of this study was to identify the selected polymorphisms in the genesis of PE. Methods: PE was identified in the second half of pregnancy, often in the latter part of the second or final trimesters, although it may occur earlier. This is a case-control study carried out in 105 cases and 100 age matched normal pregnant women (controls). We have selected three different genes: MTHFR, FVL, ACE to evaluate mutation/ polymorphisms in 677C>T, 1691G>A and I>D28005 genotypes by PCR followed by Restriction fragment length polymorphism and gel electrophoresis. Results: Statistical analysis was performed by Openepi software. Both the allele and genotype frequencies between PE cases and controls were contradictory and odds ratio with 95% confidence interval did not reveal statistical significant in either alleles or genotypes of any of the three snips. MDR analysis also failed to show the disease marker association in the PE women. It has found an interaction between MTHFR and FVL. These findings suggest the existence of population based differences in the association of candidate gene variants with PE emphasizes the importance of studying specific polymorphisms, which can be used as biomarkers uniquely for an ethnic group. Conclusion: In conclusion, the present case-control study in pregnant women appears as lack of association with the selected polymorphisms in the study population.