Abstract
The effects of thiols, ATP and CoA on the breakdown of 14C-labelled rat serum albumin by mitochondrial and lysosomal fractions from rat liver have been studied. The breakdown of serum albumin at neutral pH was negligible in comparison with the digestion at pH 5. At acid pH the hydrolysis was strongly activated by thiols like dithiothreitol, cysteine and glutathione and also to some extent by CoASH, but not by ATP. The digestion was enhaced by those treatments which rupture the lysosomal membrane. In contrast with reports in the literature, our results do not point to the presence in subcellular fractions of a proteolytic system that could be responsible for the energy dependence of intracellular protein turnover.
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