Influence of the Vehicle on the Tissue Reaction and Biomineralization of Fast Endodontic Cement

Luciana Solera Sales,Renan Dal-Fabbro,João Eduardo Gomes Filho,India Olinta De Azevedo Queiroz,Luciano Tavares Angelo Cintra,João Carlos Silos Moraes,André Pinheiro De Magalhães Bertoz,Christine Men Martins,Leopoldo Cosme-Silva,Alailson Domingos Dos Santos

doi:10.1590/pboci.2021.053

Abstract

Objective: To investigate the tissue response and the biomineralization ability of CER prepared with epoxy resin or water compared to Mineral Trioxide Aggregate (MTA) . Material and Methods: Polyethylene tubes containing materials or empty tubes for control were inserted into the subcutaneous tissues of 30 rats. After 7, 15, 30, 60, and 90 days, the rats were killed and the tubes were removed for analysis using hematoxylin-eosin staining, von Kossa staining, and under polarized light. Inflammation was graded through a score system; the thickness of the fibrous capsule was classified as thin or thick; the biomineralization ability was recorded as present or absent. The results were statistically analyzed using the Kruskal-Wallis test (p 0.05). After 30, 60, and 90 days, mild inflammation, and a thin fibrous capsule were observed in all groups (p>0.05) . Conclusion: All materials had structures positive for von Kossa and birefringent to polarized light. CER epoxy resin showed biocompatibility and biomineralization similar to CER water and MTA.

Highlights

Endodontic cements need to be biocompatible once they will be in contact with vital tissues [1,2].Mineral Trioxide Aggregate (MTA) is a repair cement that shows biocompatibility and stimulates deposition of mineralized tissue [2,3,4,5,6,7]
CER has the same indication as MTA, and it is composed of a Portland cement hydrated, water, barium sulfate, and an emulsifier [9]
During the experimental periods of analysis (30, 60 and 90 days), the inflammation significantly decreased, showing a mild infiltrate of lymphocytes and macrophages were predominant with a thin fibrous capsule for all the four groups (Figure 1: A3-A5, B3-B5, capsule; 30 (C3)-C5, D3-D5)

Summary

Introduction

Endodontic cements need to be biocompatible once they will be in contact with vital tissues [1,2]. Mineral Trioxide Aggregate (MTA) is a repair cement that shows biocompatibility and stimulates deposition of mineralized tissue [2,3,4,5,6,7]. MTA has several drawbacks, such as the handling difficulty, granular consistency and slow setting time [8]. CER has the same indication as MTA, and it is composed of a Portland cement hydrated, water, barium sulfate, and an emulsifier [9]. The cement has biological properties similar to MTA, a setting time lower than MTA, thermal expansion similar to dentin, releasing calcium and hydroxyl ions, favoring the process of tissue repair [4,5,9]

Results

Discussion

Conclusion