Influence of the TNF-α gene polymorphism - 308 G/A in the development of chronic gastritis and gastric cancer.

Abstract

Event Abstract Back to Event Influence of the TNF-α gene polymorphism - 308 G/A in the development of chronic gastritis and gastric cancer. Lluvia Yahaira S. Reyes1, Enoc M. Cortes Malagón2, Eduardo S. Castañeda Saucedo1, Rogelio Hernandez Pando3, Adolfo Roman Roman1, Dinorah N. Martínez Carrillo1 and Gloria Fernandez Tilapa1* 1 Universidad Autonoma de Guerrero, Mexico 2 Hospital juarez de Mexico, Mexico 3 Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán", Departamento de Patología, Mexico Introduction: Many of the pathogenic effects of Helicobacter pylori infection are associated with active chronic inflammation. Several polymorphisms in genes of proinflammatory cytokines have been associated with increased risk of gastric cancer. Polymorphisms in the Tumor necrosis factor alpha (TNF-α) gene are associated with differences in mRNA levels of this cytokine as well as with the magnitude of gastric inflammation, different degree of gastric acid secretion inhibition and with distinct risk of gastroduodenal disease in response to H. pylori infection. TNF-α has a potent inhibitory effect on gastric acid production and therefore influence the development of atrophy in gastric mucosa. In some populations, the TNF-α single-nucleotide polymorphism (SNP) -308 G/A is associated with risk of chronic gastritis and gastric cancer. This relationship has not been studied in population from Southwest of Mexico. Objective: To evaluate the relation between the SNP -308 G/A in the TNF-α gene with H. pylori infection in patients with chronic gastritis and gastric cancer from Southwestern of Mexico. Methods: A total of 100 patients were selected among those suffering dyspepsia symptoms or had presumptive diagnosis of gastric cancer. Endoscopic and histopathological findings were used for to establish the diagnosis of patients. Patients without anti-microbial treatment and without use of proton pump inhibitors or gastric pH neutralizers during the month prior to endoscopic procedure were included. Patients on immunosuppressive therapy or non-steroidal anti-inflammatory drugs were excluded. Two biopsies of gastric mucous of body, antrum or tumor were obtained from each patient. Total DNA was obtained from gastric biopsies by the phenol-chloroform-isoamyl alcohol technique after digestion with proteinase K. H. pylori DNA was detected by conventional PCR with primers specific to the 16S rRNA gene. Genotyping of TNF-α SNP -308 G/A was made by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: Of the total of patients included in the study, 69% were diagnosed with chronic gastritis and 31% with gastric cancer. The mean age was 48 years old for chronic gastritis cases (range 20-76 years) and 59 years (range 31- 85 years) for the gastric cancer cases. H. pylori DNA was detected in 40% of chronic gastritis patients and 52% in patients with gastric cancer. The genotype distribution of the TNF-α SNP -308 G/A between patients infected with H. pylori was G/G 79%, G/A 6% and A/A 15%, and G/G 78%, G/A 13% and A/A 9% among uninfected patients. The genotypic distribution in chronic gastritis was G/G 80%, G/A 14% and A/A 6% and G/G 70%, G/A 3% and A/A 27% in gastric cancer. The allelic frequencies were 82% G and 18% A in chronic gastritis and 71% G and 29% A in gastric cancer. The allelic frequencies patients infected with H. pylori was 54% G and 14% A, and 31% G and 9% A in unaffected patients. The -308 A/A genotype was associated with risk of gastric cancer (p= 0.05, OR = 4.1, 95% Cl = 0.9-17.6). Conclusions: In population from Southwest Mexico, the genotype -308 A/A of the TNF-α SNP -308 G/A increase the risk for developing gastric cancer. The results of this study support the hypothesis that H. pylori infection and host genetic factors, such as the TNF-α SNP -308 G/A, can play an important role in the development of gastric cancer in Southwestern Mexican population. References -Amieva, M.R., El–Omar, E.M., 2008. Host-Bacterial Interactions in Helicobacter pylori Infection. Gastroenterology 134, 306–323. -Aguillón, J.C., Cruzat, A., Cuenca, J., Cuchacovich, M. 2002. El polimorfismo genético del factor de necrosis tumoral alfa como factor de riesgo en patología.Revista Médica de Chile, 130. 1043-1050. Fox, J.G., Wang, T.C., 2007. Inflammation, atrophy, and gastric cancer. J. Clin. Invest. 117, 60–69. -He, C., Chen, M., Liu, J., Yuan, Y., 2013. Host genetic factors respond to pathogenic step-specific virulence factors of Helicobacter pylori in gastric carcinogenesis. Mutat. 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Keywords: TNF-α, Helicobacter pylori, gastric cancer, Polymorphism, Genetic, proinflammatory cytokines Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Infectious and parasitic diseases Citation: Reyes LS, Cortes Malagón EM, Castañeda Saucedo ES, Hernandez Pando R, Roman Roman A, Martínez Carrillo DN and Fernandez Tilapa G (2015). Influence of the TNF-α gene polymorphism - 308 G/A in the development of chronic gastritis and gastric cancer. . Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00148 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 May 2015; Published Online: 14 Sep 2015. * Correspondence: Dr. Gloria Fernandez Tilapa, Universidad Autonoma de Guerrero, Chilpancingo de los Bravo, Guerrero, 39087, Mexico, gferti@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Lluvia Yahaira S Reyes Enoc M Cortes Malagón Eduardo S Castañeda Saucedo Rogelio Hernandez Pando Adolfo Roman Roman Dinorah N Martínez Carrillo Gloria Fernandez Tilapa Google Lluvia Yahaira S Reyes Enoc M Cortes Malagón Eduardo S Castañeda Saucedo Rogelio Hernandez Pando Adolfo Roman Roman Dinorah N Martínez Carrillo Gloria Fernandez Tilapa Google Scholar Lluvia Yahaira S Reyes Enoc M Cortes Malagón Eduardo S Castañeda Saucedo Rogelio Hernandez Pando Adolfo Roman Roman Dinorah N Martínez Carrillo Gloria Fernandez Tilapa PubMed Lluvia Yahaira S Reyes Enoc M Cortes Malagón Eduardo S Castañeda Saucedo Rogelio Hernandez Pando Adolfo Roman Roman Dinorah N Martínez Carrillo Gloria Fernandez Tilapa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.