Influence of the Temperature and Cell Line on the Adsorption and Replication of Five Fish Rotaviruses.

Abstract

The infectivity of five fish rotaviruses was assayed on 4 fish and two mammalian cell lines at different temperatures. These viruses were isolated recently from turbot (TRV), smelt (SRV), striped bass (SBR) and Atlantic salmon (ASR and HBR). The two mammalian lines, MA 104 and MDBK, were not susceptible to any of the viruses assayed, although viruses were adsorbed by the cells at certain temperatures. Of the four fish cell lines employed (CHSE-214, FHM, EPC and BB), only FHM cell failed to support the the growth of all the viruses. The EPC cells were susceptible, but the titers were low (l105.5 TCID50/ml). Although the BB cells proved to be very useful for the replication of SRV, the Atlantic salmon rotavirus failed to grow in this cell line. The CHSE-214 cell is the optimal cell line for all these rotaviruses because they produced relatively high titers (between 106.25 and 109.25 TCID50/ml) when the cells were incubated at 15°C.