Influence of the strain of rats on the induction of hexachlorobenzene induced porphyria

Rosa Wainstok De Calmanovici,Silvia C.Billi De Catabbi,Carmen A Aldonatti,Leonor C.San Martín De Viale

doi:10.1016/0020-711x(89)90361-3

Rosa Wainstok De Calmanovici, Silvia C.Billi De Catabbi + Show 2 more

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https://doi.org/10.1016/0020-711x(89)90361-3

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Abstract

1. 1. The present work undertakes a comparative study on the hexachlorobenzene (HCB) porphyria induction in female rats of Wistar and CHBBTHOM strains. The purpose was to characterize the CHBBTHOM strain with respect to the haem metabolic pathway, its regulatory mechanisms and its response to foreign drugs. 2. 2. After 7 weeks of treatment it was observed that the hepatic porphyrins increased 140 times, ALA-synthase 4 times and PCL was 73% inhibited in the Wistar strain. 3. 3. On the other hand the animals of CHBBTHOM strain showed lesser alteration on these parameters; hepatic porphyrins increased only 3-fold, ALA-synthase 1.7-fold and PCL was only 22% inhibited. 4. 4. Total iron liver content was nearly equal in both strains of rats. 5. 5. The results obtained would indicate that the lower susceptibility of the CHBBTHOM strain to acquire porphyria does not seem to be due to either: (1) congenital alterations of any parameters of the haem metabolic pathway, since the behaviour of normal animals from both strains was similar; or (2) a lower hepatic iron content in such animals. 6. 6. These findings would suggest that the differential response to HCB to this strain would be looked for in another metabolic pathway, such as that involved in the metabolization process of the toxic.

Highlights

Of ALA, PBG and porphyrins significantly exceeded the control values in the Wistar strain; instead the corresponding levels in the CHBBTHOM animals remained within normal range
The degree of inhibition was only 22 and 55%, respectively. These results indicate that these two enzymatic aetivities were affected to a different extent in both strains, after 7 weeks of fungicide treatment, the Wistar strain being more susceptible than the CHBBTHOM to the action of HCB
The results of the present work show that a great difference, in the intensity and in the time necessary to produce a disturbance in the haem metabolism, exist between the two strains of rats

Summary

Introduction

The purpose was to characterize the CHBBTHOM strain with respect to the haem metabohe pathway, its regulatory mechanisms and its response to foreign drugs. 2. After 7 weeks of treatm~t it was observed that the hepatic porphyrins increased 140 times, ALA-synthase 4 times and PCL was 73% inhibited in the Wistar strain. 3. On the other hand the animals of CHBBTHOM strain showed lesser alteration on these parameters; hepatic porphyrins increased only 3-fold, ALA-synthase 1.7-fold and PCL was only 22% inhibited. The results obtained would indicate that the lower susceptibility of the CHBBTHOM strain to acquire porphyria does not seem to be due to either: (1) congenital alterations of any parameters of the haem metabolic pathway, since the behaviour of normal animals from both strains was similar; or (2) a lower hepatic iron content in such animals. These findings would suggest that the differential response to HCB to this strain would be looked for in another metabolic pathway, such as that involved in the metabolization process of the toxic

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