Abstract
The sensitivity of Mycobacterium fortuitum at various growth stages to electric field pulses has been investigated in order to transform the cells by electroporation. In contrast to older cells, early- and mid-exponential growing cells are very field sensitive and, simultaneously, transformable to a relatively high degree. At an electric field strength E = 7 kV/cm and a pulse duration τ ≈ 13 ms (one pulse) an optimum transformation rate of about 2 × 10 4 transformants/μg DNA was observed. On the basis of the different fast change of transformation rate and field sensitivity during cell growth we suppose a remarkable influence of biochemical changes in the cell wall composition on the efficiency of the electric field mediated transformation.
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