Abstract
Lipase from Pseudomonas fluorescens (Amano AK) was immobilized using the sol-gel entrapment technique and sol-gel entrapment combined with adsorption on a solid support (Celite 545). The sol-gel matrices were obtained using as precursors ternary mixtures of tetramethoxysilane and trimethoxysilanes substituted with phenyl or vinyl groups, at different molar ratios, and l-octyl-3-methyl-immidazolium tetrafluoroborate as additive. The obtained enzymatic preparates were efficient biocatalysts in the acylation reaction of racemic secondary alcohols. Several methods were employed for the characterization of the resulting bioactive materials, in order to correlate the catalytic performance with the morphological and physico-chemical properties of the matrix: Fourier transform infrared spectroscopy (FTIR), BET-analysis of specific surface and porosity, laser scanning confocal microscopy (LSCM), scanning electron microscopy (SEM), atomic force microscopy (AFM) and thermogravimetric analysis (TGA). An extensive stu...
Highlights
Biotransformations are already being used industrially to manufacture a wide range of products, including drugs, agricultural chemicals, organics, fine chemicals and plastics
Lipase from Pseudomonas fluorescens was immobilized by sol-gel entrapment and sol-gel entrapment combined with adsorption, using the methods previously applied for Candida antarctica B lipase [19]
Numerous instrumental techniques are available for the investigation and characterization of hybrid organicinorganic matrices obtained by the sol-gel method
Summary
Biotransformations are already being used industrially to manufacture a wide range of products, including drugs, agricultural chemicals, organics, fine chemicals and plastics. Pharmaceutical companies, as well as chemical and food industries using biocatalysts, are developing, due to the increasing demand for new, efficient and low production costs [1,2,3,4]. Lipases (E.C. 3.1.1.3.) are remarkable industrial biocatalysts due to their specificity, thermal stability and tolerance to organic solvents. Native lipases have limited stability, so immobilization is essential for their usage in an industrial setup. Immobilized lipases show a variety of properties, such as increased catalytic activity, temperature and solvent tolerance [7, 8], reusability and ease of the downstream process [9,10,11,12]
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