Abstract
In this research, we first determined the three most significant nutrient factors affecting haloalcohol dehalogenase HheC production by Escherichia coli P84A/MC1061. These were glycerol, yeast extract, and ammonium sulfate. The steepest ascent method was then applied to obtain the optimal design intervals of the three factors. An application of center composite design was used, and the ingredients of the optimized medium were 1.8 g l−1 glycerol, 48 g l−1 yeast extract, 2.2 g l−1 ammonium sulfate, 5 g l−1 compound phosphate, 1 g l−1 magnesium sulfate, and 1.19 × 10−5 g l−1 ferric sulfate. The enzyme activity reached 109,365 U ml−1 under the most favorable conditions, which is a 277.7 % increase compared with the control group. Our study of cellular respiration parameters (oxygen uptake rate and carbon dioxide emission rate) revealed that the metabolic activity of the strain was strongly promoted under these optimal nutrient conditions and that yeast extract had a positive effect on respiratory intensity and the expression levels of HheC.
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