Influence of the ligand donor atoms on the in vitro stability of rhenium(I) and technetium (I)-99m complexes with pyrazole-containing chelators: Experimental and DFT studies

Abstract

The new pyrazole-containing ligand 3,5-Me 2pz(CH 2) 2S(CH 2) 2COOH ( L 1 H) was synthesized and used to prepare the complexes fac-[M(κ 3-L 1)(CO) 3] (M = Re (1), 99mTc( 1a)), which were obtained in high yield albeit with a low specific activity in the case of 99mTc. The X-ray diffraction analysis of 1 confirmed that L 1 coordinates to the metal as monoanionic and through a (N,S,O) donor atom set. Challenge experiments of 1a against cysteine and histidine showed that this complex suffers considerable transchelation in vitro. This contrasts with the behavior exhibited by the related complex fac-[ 99mTc(κ 3- L 2 )(CO) 3] ( 2a) ( L 2 = 3,5-Me 2pz-(CH 2) 2NH-CH 2-COO), anchored by a (N 2O)-tridentate ligand. Biodistribution studies of 1a and 2a in mice indicated that both compounds have a relatively similar biological profile. Nevertheless, the fastest blood clearance and minor hepatic retention found for 2a has shown that this complex is more adequate to be further explored in radiopharmaceutical sciences. DFT calculations (ADF program) were performed for these neutral complexes and related cationic M(I) (M = Re, Tc) tricarbonyl complexes anchored by pyrazole-containing ligands, in order to have a better understanding of the influence of the donor atom set (N,N,O vs. N,O,S; N,N,N vs. N,N,S vs. N,S,S) on their in vitro stability. The differences of the calculated binding energies are not significant, suggesting that the in vitro behavior of these Re(I)/Tc(I) tricarbonyl complexes is not determined by thermodynamic factors.