Abstract
Abstract1‐ (2,3‐dideoxy‐β‐D‐erythro‐hexopyranosyl) thymine was used at several positions as nucleoside substitute in the synthesis of oligonucleotides using the H‐phosphonate chemistry. A 13‐mer with one hexose nucleoside substitution at each end had equivalent hybridization properties as compared to the non‐modified 13‐mer. However, addition of a second analogue at each end or modification in the middle of a 13‐mer caused a sharp decrease in melting temperature. Substitution at the 3′‐end with two nucleoside analogues was necessary to withstand the action of snake venom phosphodiesterase.
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