Influence of the extract of wen-pi-tang on memory function of mice and SOD and MDA in brain

Abstract

Objective To explore the influence of the extract of wen-pi-tang on the memory and SOD and MDA of normal mice and cerebral ischemia reperfusion mice. Method The mice were divided into normal group, sham operation group, model group, aqueous extract and alcohol extract of wen-pi-tang (1.0 g/kg, 0.5 g/kg, 0.25 g/kg). Model mice with cerebral ischemia reperfusion were made by blocking bilateral common carotid artery. The influence on memory behavior of normal mice and mice with cerebral ischemia reperfusion were tested by gavage for 10 days with extract of wen-pi-tang through step-down and step-through tests.Then SOD activity and content of the MDA were tested. Result In step-down test, compared with normal group, extract of wen-pi-tang with all doses made normal mice’ latency longer, error frequency fewer, with high does clearest(P<0.05)(latency: (187.17±99.00)s, (270.73±44.73)s, (260.45±63.78)s, error frequency(1.75±1.71)times, (0.45±0.69)times , (0.75±0.97)times); in step-through test, compared with normal group, aqueous extract with high and middle doses and alcohol extract with longe doses could made latency of cerebral ischemia reperfusion mice longer and error frequency fewer( latency(157.15±84.91)s, (250.63±58.98)s, (251.12±78.22)s, (245±84.82)s, error frequency(2.15±1.22)times, (0.75±0.89)times, (0.75±1.16) times, (0.625±0.92)times)(P<0.05); extract of wen-pi-tang with high and middle does increased SOD activity obviously compared with normal group(P<0.05), and the content of MDA was obviously lower compared with normal group(P<0.05, P<0.01). Conclusion Extracts of wen-pi-tang can improve the memory function of normal mice, and the memory of cerebral ischemia reperfusion mice.The mechanism can be related to the increase of SOD activity and the decrease of the content of MDA. Key words: Wen-pi-tang; Cerebral ischemia; Reperfusion; Memory; SOD; MDA