Abstract
Analysis of trace elements in blood serum can be an important tool for monitoring micronutrient supply in stallions in order to maintain reproductive health. In this regard, the purpose of this study was to determine the relationship between the concentrations of macro- and microelements in the blood serum of stud stallions with the qualitative characteristics of fresh sperm and sperm after cryopreservation and thawing. The studies were carried out on purebred Arabian stallions (n=50). As a biomaterial for studies, we used blood serum, fresh and thawed sperm after cryopreservation. The elemental composition of seminal plasma was determined by 25 chemical elements Co, Cr, Cu, Fe, I, Mn, Se, Zn Ca, K, Mg, P, B, Li, Si, V Na, Ni, Sn, Hg, Sr, Al, As, Cd, Pb) by ICP-DRC-MS methods. It was found that the concentrations of Mg and Co in the blood serum positively correlate with the qualitative characteristics of the sperm of Arabian purebred stallions. Wherein, the optimal range of concentrations of Mg in the blood serum, typical for stallions with relatively high indicators of sperm quality, is 22.64 to 27.05 μg/g; Co – 0.001 to 0.0015 μg/g.
Highlights
Along with development of new reproductive technologies in animal husbandry, a search is underway for methods to recover the natural fertility of animals and humans
The purpose of this study was to determine the relationship between the concentrations of macro- and microelements in the blood serum of stud stallions with the qualitative characteristics of fresh sperm and sperm after cryopreservation and thawing
The results obtained in our study demonstrated a positive trend in the indicators of sperm quality as the concentration of Co in the blood serum increased (Table 2)
Summary
Along with development of new reproductive technologies in animal husbandry, a search is underway for methods to recover the natural fertility of animals and humans. Copper (Cu) is an essential component of many metalloenzymes such as superoxide dismutase, ceruloplasmin and lysine oxidase, which are critical for antioxidant processes in sperm. It affects the progressive motility, viability, membrane integrity and prevents DNA damage after sperm dilution and cryopreservation [4, 5, 6].
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