Influence of the complex of vitamins Pikovit in culture in vitro on the growth and development of potato microplants

Yulia Fedorova,Margarita Zaitseva,Larisa Fedorova

doi:10.1051/bioconf/20224302004

Yulia Fedorova, Margarita Zaitseva + Show 1 more

Open Access

https://doi.org/10.1051/bioconf/20224302004

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Abstract

In the conditions of the North-West zone of Russia, studies of Pikovit vitamin complex preparation were carried out. Since the main way to obtain a healthy seed is clonal micropropagation. Therefore, in the course of the work, the process and method of clonal micropropagation of Russian potato varieties using new preparations were improved, the selection of nutrient media at all stages of cultivation was carried out with the Pikovit preparation at the concentration of 0.5 to 1.5 mg/l. As a result, it was found that use of the Pikovit vitamin complex has a positive effect on the ontogenesis of potato microplants. To obtain well-developed microplants of the early variety Liga, the most optimal concentration is 1.5 mg/l. For other studied varieties of the mid-ripening group Aurora and Gusar, the nutrient medium with the addition of vitamins at the concentration of 1.0 mg/l was optimal.

Highlights

One of the main agricultural crops is potato, its demand among the population is increasing every year
To improve the technology of reproduction of the source material, we studied selection of the optimal concentration of the Pikovit vitamin complex, which has a positive effect on production of high-quality seed material [10, 12]
The studies were carried out according to the methods for carrying out work with tissue culture and clonal micropropagation of potatoes, methods for potatoes developed by the All-Russian Research Institute of Potato Farming and the Department of Biotechnology of the Moscow Agricultural Academy

Summary

Introduction

One of the main agricultural crops is potato, its demand among the population is increasing every year. The potential of modern potato varieties is up to 80 t/ha and more, but, in most farms, the yield of this crop does not exceed 20 t/ha. This is primarily due to the low quality of the used seed [1]. In the context of a lack of production of domestic seed potatoes, one of the main ways to increase the volume of cultivation of the original healthy seed material is development of new methods for intensifying the process of reproduction of seed potato seed at the initial stages of seed production [16]

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