INFLUENCE OF THE COMPLEX BACTERIAL PREPARATION AZOGRAN ON THE DEVELOPMENT OF SPROUTS OF CULTIVATED PLANTS

Abstract

Objective. To investigate the effect of seed treatment of cultivated plants with the complex bacterial preparation Azogran (based on Bacillus subtilis IMB B-7023 and Azotobacter vinelandii IMB B-7076) on the initial stages of ontogenesis under laboratory conditions. Methods. Microbiological (cultivation of microorganisms was carried out under periodic culture conditions — A. vinelandii IMB B-7076 in Ashby liquid digest medium, Bacillus subtilis IMB B-7023 — in a mineral medium with glucose; the number of viable cells in suspensions was determined by the method of serial dilutions followed by sowing on the surface of the relevant agarized media), physiological (with different dilutions of biopreparation suspensions for seed inoculation), statistical. Results. Bacterization of Ukraina variety buckwheat seeds, Podolianka variety winter wheat and Marharyta variety common tare with a liquid complex bacterial preparation Azogran had a positive effect on germination energy, germination, sprout length and their weight compared to the control (water treatment). The best results at the initial stages of plant ontogenesis were found for buckwheat and wheat. The positive effect of inoculation was more pronounced when diluting the biopreparation suspension with water in ratios of 1:10 and 1:100, which indicates intensive production of biologically active substances by bacteria. Treatment of seeds of different corn varieties with an aqueous suspension of the granular complex bacterial preparation Azogran had a positive effect on the development of sprouts, and also contributed to the reduction of their damage by phytopathogenic micromycetes. Conclusion. The complex bacterial preparation Azogran has a positive effect on the energy of germination, germination and the weight of sprouts of the investigated plant species, reduces the degree of seed damage by micromycetes and is recommended for the treatment of seeds of agricultural plants before sowing in the soil.