Influence of the brain ischemia-reperfusion on the condition of the Bcl-2-dependent antiapoptotic mechanisms in hippocampus of rats with diabetes mellitus

Abstract

In the animals without diabetes mellitus (DM), after 20 min ischemia/1 hour reperfusion, the activity of Bcl-2 antiapoptotic mechanisms increases in the hippocampal fields CA1, CA2, CA4, and reduces – in the field CA3. On the 12th day of the postischemic period, antiapoptotic activity in fields CA1 and CA4 returns to the level of activity in animals of the control group; in field CA2 it decreases compared to the controls; and, in field CA3 – depression of the antiapoptotic activity becomes deeper. The four-month DM activates Bcl-2 antiapoptotic mechanisms in fields CA3 and CA; suppresses them in the CA1 field and does not affect them – in field CA4. In rats with four-month diabetes, in early postischemic period, the activity of Bcl-2 antiapoptotic processes increases in fields CA1 and CA4, and reduces – in fields CA2 and CA3. On the 12th day of ischemia-reperfusion period in rats with diabetes, the Bcl-2 antiapoptotic activity returns to level of activity in diabetic rats without disturbing the cerebral blood flow in field CA1; decreases – in the CA4, and undergoes even greater depression than in the early period, in fields CA2 and CA3.