Influence of the antimicrotubular agents, colchicine and vinblastin, on the uptake of americium by rat liver, kidneys, and skeleton

Abstract

The influence of pretreatment with colchicine and vinblastin sulfate, administered ip at dosages of 2.5 μmol/kg on the uptake of im or iv injected 241Am by rat liver, kidneys, and skeleton was investigated. Both agents reduced the nuclide uptake by liver and increased the 241Am contents in kidneys and skeleton. The effect was dependent on the time interval between pretreatment and nuclide injection as well as on the dose of the agents. The minimum effective dose was ∼0.5 μmol/kg. A thousand times higher po dose of carbon tetrachloride was less effective than the highest colchicine dose. Lumicolchicine (2.5 μmol/kg) did not increase the 241Am skeleton and kidney contents and was less effective in reducing the hepatic content than colchicine or vinblastin. At the light microscopy level, livers of the rats appeared normal after the standard colchicine dose (1 mg/kg), but concentrations of four enzymes, which may indicate damage to the liver, were significantly raised.